UseCases_ArguminSci_Discourse  

PubMed:12895502 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T1 0-147 DRI_Background denotes Neocortical neurons cultured from mice with expanded CAG repeats in the huntingtin gene: unaltered vulnerability to excitotoxins and other insults.
T2 148-284 DRI_Challenge denotes Glutamate-mediated excitotoxicity might contribute to the pathogenesis of Huntington's disease and other polyglutamine repeat disorders.
T3 285-503 DRI_Approach denotes We used murine neocortical cultures derived from transgenic and knock-in mice to test the effect of expression of expanded polyglutamine-containing huntingtin on neuronal vulnerability to excitotoxins or other insults.
T4 504-800 DRI_Background denotes Neurons cultured from mice expressing either a normal length (Hdh(Q20)) or expanded (Hdh(Q111)) CAG repeat as a knock-in genetic alteration in exon one of the mouse Hdh gene [Hum Mol Genet 8 (1999) 115] had similar vulnerability to N-methyl-D-aspartate (NMDA) and kainate-mediated excitotoxicity.
T5 801-920 DRI_Background denotes These neurons also exhibited similar vulnerability to oxidative stress (24 h exposure to 10-100 microM paraquat or 1-10
T6 921-937 Token_Label.OUTSIDE denotes microM menadione
T7 937-996 DRI_Background denotes ), apoptosis (48 h exposure to 30-100 nM staurosporine or 1
T8 997-1015 Token_Label.OUTSIDE denotes microM dizocilpine
T9 1016-1076 DRI_Background denotes maleate (MK-801) and proteasome inhibition (48 h exposure to
T10 1077-1096 Token_Label.OUTSIDE denotes 0.3-3 microM MG-132
T11 1096-1098 DRI_Background denotes ).
T12 1099-1255 DRI_Background denotes Neocortical neurons cultured from mice transgenic for an expanded CAG repeat-containing exon 1 of the human HD gene (Mangiarini et al., 1996, R6/2 line) and
T13 1256-1281 Token_Label.OUTSIDE denotes non-transgenic littermate
T14 1282-1366 DRI_Background denotes controls also had similar vulnerability to NMDA and kainate-mediated excitotoxicity.
T15 1367-1566 DRI_Outcome denotes These observations suggest that expression of expanded polyglutamine-containing huntingtin does not acutely alter the vulnerability of cortical neurons to excitotoxic, oxidative or apoptotic insults.