| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-116 |
DRI_Approach |
denotes |
Expression and activation of matrix metalloproteinase-2 and -9 in rat brain after transient focal cerebral ischemia. |
| T2 |
117-247 |
DRI_Approach |
denotes |
Matrix metalloproteinases (MMPs) degrade the extracellular matrix and carry out key functions during development and after injury. |
| T3 |
248-411 |
DRI_Background |
denotes |
By means of zymography, Western blot and immunohistochemistry, we studied MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in rat brain after focal cerebral ischemia. |
| T4 |
412-533 |
DRI_Background |
denotes |
The control rat brain showed constitutive MMP-2 and, to a lesser extent, MMP-9, which were mainly present as prozymogens. |
| T5 |
534-680 |
DRI_Background |
denotes |
MMP-2 protein was located in the cell body of neurons, glia, and endothelium, whereas MMP-9 was associated to neurons and myelinated fibre tracts. |
| T6 |
681-1054 |
DRI_Unspecified |
denotes |
Ischemia greatly increased MMP activation in two temporal waves, in the first one, MMP-9 protein was induced from 4 h to 4 days, and also a small and short-lasting increase in MMP-2 was detected at 4 h. The second wave showed a massive increase in MMP-2 protein expression and activation by day 4, which was compatible with abundant MMP-2 in reactive microglia/macrophages. |
| T7 |
1055-1111 |
DRI_Outcome |
denotes |
Our results are compatible with progressive induction of |
| T8 |
1112-1125 |
Token_Label.OUTSIDE |
denotes |
MMP-9 proform |
| T9 |
1125-1169 |
DRI_Outcome |
denotes |
, likely in neurons, shortly after ischemia. |
| T10 |
1170-1439 |
DRI_Outcome |
denotes |
For MMP-2, the results suggest a discrete production immediately after reperfusion, while a very enhanced expression and activation of MMP-2 attributable to microglia/macrophages occurs on day 4, and it might contribute to the phagocytic action of these reactive cells. |
