UseCases_ArguminSci_Discourse  

PubMed:11222998 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T1 0-161 DRI_Background denotes Immunohistochemistry of matrix metalloproteinases in reperfusion injury to rat brain: activation of MMP-9 linked to stromelysin-1 and microglia in cell cultures.
T2 162-212 DRI_Unspecified denotes Reperfusion damages the blood-brain barrier (BBB).
T3 213-362 DRI_Approach denotes Matrix metalloproteinases (MMPs) are associated with the opening of the BBB, but their cellular localization and activation mechanisms are uncertain.
T4 363-512 DRI_Approach denotes We used immunohistochemistry to determine the cellular localization of the MMPs in reperfused rat brain, and cell cultures to study their activation.
T5 513-658 DRI_Background denotes Spontaneously hypertensive rats (SHR) had a 90 min middle cerebral artery occlusion (MCAO) followed by reperfusion for times from 3 h to 21 days.
T6 659-783 DRI_Approach denotes Frozen sections were immunostained with antibodies to gelatinase A (MMP-2), stromelysin-1 (MMP-3), and gelatinase B (MMP-9).
T7 784-817 DRI_Background denotes Sham-operated control rats showed
T8 818-838 Token_Label.OUTSIDE denotes MMP-2 immunostaining
T9 839-885 DRI_Background denotes in astrocytic processes next to blood vessels.
T10 886-923 DRI_Background denotes After 3 h of the onset of reperfusion
T11 924-944 Token_Label.OUTSIDE denotes MMP-2 immunostaining
T12 945-969 DRI_Background denotes increased in astrocytes.
T13 970-1024 DRI_Unspecified denotes At 24 h immunoreactivity for MMP-3 and MMP-9 appeared.
T14 1025-1030 REPLACED denotes MMP-3
T15 1031-1043 Token_Label.OUTSIDE denotes co-localized
T16 1044-1107 DRI_Unspecified denotes with activated microglia (Ox-42+) and ischemic neurons (NeuN+).
T17 1108-1113 REPLACED denotes MMP-9
T18 1114-1128 Token_Label.OUTSIDE denotes immunostaining
T19 1129-1193 DRI_Approach denotes was seen at 48 h in endothelial cells, neutrophils, and neurons.
T20 1194-1291 DRI_Background denotes At 5 and 21 days intense MMP-2 staining was seen in reactive astrocytes around the ischemic core.
T21 1292-1407 DRI_Background denotes Studies of activation of the MMP were done in lipopolysaccharide (LPS)-stimulated astrocyte and microglia cultures.
T22 1408-1466 DRI_Background denotes Stimulated astrocytes produced an activated form of MMP-2.
T23 1467-1520 DRI_Background denotes When microglia were stimulated, they activated MMP-9.
T24 1521-1590 DRI_Background denotes Immunostaining showed MMP-3 in cultures of enriched microglial cells.
T25 1591-1710 DRI_Outcome denotes The hydroxymate-type, MMP inhibitor, BB-1101, blocked the activation of MMP-2 and MMP-9 by LPS in mixed glial cultures.
T26 1711-1831 DRI_Challenge denotes We propose that MMP-2 is normally present in astrocytic end feet, and that during ischemia MMP-9 and MMP-3 are produced.
T27 1832-1890 DRI_Approach denotes MMP-3 in microglia/macrophages may be activating proMMP-9.
T28 1891-2069 DRI_Outcome denotes Our results show that a differential expression of MMPs by astrocytes, microglia, and endothelial cells at the blood vessels is involved in the proteolytic disruption of the BBB.