| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-176 |
DRI_Background |
denotes |
Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas. |
| T2 |
177-368 |
DRI_Approach |
denotes |
Matrix metalloproteinases (MMPs) have been implicated as important factors in gliomas since they may both facilitate invasion into the surrounding brain and participate in neovascularization. |
| T3 |
369-631 |
DRI_Approach |
denotes |
We have tested the hypothesis that deregulated expression of gelatinase-A or B, or an activator of gelatinase-A, MT1-MMP, may contribute directly to human gliomas by quantifying the expression of these MMPs in 46 brain tumour specimens and seven control tissues. |
| T4 |
632-839 |
DRI_Background |
denotes |
Quantitative RT-PCR and gelatin zymography showed that gelatinase-A in glioma specimens was higher than in normal tissue; these were significantly elevated in low grade gliomas and remained elevated in GBMs. |
| T5 |
840-969 |
DRI_Background |
denotes |
Gelatinase-B transcript and activity levels were also higher than in normal brain and more strongly correlated with tumour grade. |
| T6 |
970-1093 |
DRI_Approach |
denotes |
We did not see a close relationship between the levels of expression of MT1-MMP mRNA and amounts of activated gelatinase-A. |
| T7 |
1094-1233 |
DRI_Background |
denotes |
In situ hybridization localized gelatinase-A and MT1-MMP transcripts to normal neuronal and glia, malignant glioma cells and blood vessels. |
| T8 |
1234-1415 |
DRI_Outcome |
denotes |
In contrast, gelatinase-B showed a more restricted pattern of expression; it was strongly expressed in blood vessels at proliferating margins, as well as tumour cells in some cases. |
| T9 |
1416-1523 |
DRI_Outcome |
denotes |
These data suggest that gelatinase-A, -B and MT1-MMP are important in the pathophysiology of human gliomas. |
| T10 |
1524-1705 |
DRI_Challenge |
denotes |
The primary role of gelatinase-B may lie in remodelling associated with neovascularization, whereas gelatinase-A and MT1-MMP may be involved in both glial invasion and angiogenesis. |
