| T3 |
0-785 |
RESULTS |
denotes |
In nasal, tracheal, and bronchial explants, we noticed a 10- to 100-fold lower replication of R2-HK compared with R1-HK. In the lung explants, the viruses replicated with comparable efficiency. These observations correlated with the known expression level of Siaα2,3-galactose in these tissues. In the pathogenesis study, virus titers in the respiratory part of the nasal mucosa, the trachea, and the bronchus were in line with the ex vivo results. R2-HK replicated less efficiently in the lungs of pigs than R1-HK, which contrasted with the explants results. R2-HK also showed a pronounced tropism for the olfactory part of the nasal mucosa. Transmissibility experiments revealed that pig-to-pig transmission was abrogated when the virus obtained Siaα2,3-galactose binding preference. |
