| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
154-418 |
OBJECTIVE |
denotes |
We provide the necessary tools to study the biology of bone metastasis using renal cell carcinoma as a model. A relevant renal cell carcinoma cell line developed from a human bone metastasis is described and an experimental model in the nude mouse was established. |
| T2 |
442-833 |
METHODS |
denotes |
The novel cell line RBM1 was developed from bone metastasis from a patient with renal cell carcinoma. In vitro methods used to study the cell line included karyotype analysis, reverse-transcriptase polymerase chain reaction, ribonuclease protection assay and Western blot analysis. An intratibial injection model in the nude mouse resulted in bone lesions similar to those in human patients. |
| T3 |
843-1395 |
RESULTS |
denotes |
Chromosomal analysis of this cell line revealed characteristic cytogenetic abnormalities common in renal cell carcinoma. RBM1 cells expressed parathyroid hormone parathyroid hormone related peptide, interleukin-6 and macrophage colony-stimulating factor, which are cytokines involved in osteoclast activation and subsequent bone resorption. Western blot analysis revealed the presence of high levels of epidermal growth factor receptor and c-MET. Reproducible osteolytic lesions developed in the nude mouse after injecting 1 x 106 cells into the tibia. |
| T4 |
1409-1534 |
CONCLUSIONS |
denotes |
This cell line and animal model may allow further study of the biology and mechanism of renal cell carcinoma bone metastasis. |
