PubMed_ArguminSci  

PubMed:7579328 JSONTXT 15 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 75-181 DRI_Background denotes MZF-1 is a C2H2 zinc finger gene encoding a putative transcriptional regulator of myeloid differentiation.
T2 182-349 DRI_Approach denotes The MZF-1 protein contains 13 C2H2 zinc fingers arranged in bipartite DNA binding domains containing zinc fingers through 4 and, in the carboxy-terminus, 5 through 13.
T3 350-448 DRI_Approach denotes We previously identified the DNA consensus binding site recognized by the two DNA binding domains.
T4 449-591 DRI_Approach denotes To assess the transcription regulatory function of MZF-1, the full-length MZF-1 coding region was fused to the DNA binding domain of the yeast
T5 611-612 DRI_Approach denotes .
T6 613-841 DRI_Approach denotes The expression vector was cotransfected with the chloramphenicol acetyl transferase (CAT) reporter gene regulated by the thymidine kinase promoter containing GAL4 DNA binding sites into NIH 3T3, 293, K562, and Jurkat cell lines.
T7 842-961 DRI_Approach denotes MZF-1 represses CAT reporter gene expression via GAL4 binding sites in the nonhematopoietic cell lines NIH 3T3 and 293.
T8 962-1068 DRI_Background denotes In contrast, MZF-1 activates CAT reporter gene expression in the hematopoietic cell lines K562 and Jurkat.
T9 1069-1209 DRI_Background denotes The MZF-1 binding sites are present in the promoters of several genes expressed during myeloid differentiation, including the CD34 promoter.
T10 1210-1352 DRI_Background denotes MZF-1 transcriptional regulation of this physiologically relevant promoter was assessed in both hematopoietic and nonhematopoietic cell lines.
T11 1353-1475 DRI_Approach denotes Recombinant MZF-1 protein specifically binds to the consensus binding sites in the CD34 promoter in mobility shift assays.
T12 1476-1645 DRI_Approach denotes MZF-1 expression vectors were cotransfected with the luciferase reporter plasmids regulated by the CD34 promoter into both nonhematopoietic and hematopoietic cell lines.
T13 1646-1816 DRI_Background denotes As with the heterologous DNA binding domain, MZF-1 represses reporter gene expression in nonhematopoietic cell lines and activates expression in hematopoietic cell lines.
T14 1817-1934 DRI_Approach denotes Activation of CD34 expression in hematopoietic cell lines is dependent on the presence of intact MZF-1 binding sites.
T15 1935-2153 DRI_Approach denotes The cell type-specific regulation of the CD34 promoter by MZF-1 suggests the presence of tissue-specific regulators/adapters or differential MZF-1 modifications that determine MZF-1 transcriptional regulatory function.