PubMed_ArguminSci  

PubMed:31341019 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T1 154-319 DRI_Background denotes The triple-negative phenotype is the most prevalent form of human breast cancer worldwide and is characterized by poor survival, high aggressiveness, and recurrence.
T2 320-483 DRI_Background denotes Microvesicles (MV) are shredded plasma membrane components and critically mediate cell-cell communication, but can also induce cancer proliferation and metastasis.
T3 484-725 DRI_Background denotes Previous studies have revealed that protease-activated receptor 2 (PAR2) contributes significantly to human triple-negative breast cancer (TNBC) progression by releasing nano-size MV and promoting cell proliferation, migration, and invasion.
T4 726-829 DRI_Background denotes MV isolated from highly aggressive human TNBC cells impart metastatic potential to nonmetastatic cells.
T5 830-1000 DRI_Background denotes Over-expression of microRNA221 (miR221) has also been reported to enhance the metastatic potential of human TNBC, but miR221's relationship to PAR2-induced MV is unclear.
T6 1001-1342 DRI_Outcome denotes Here, using isolated MV, immunoblotting, quantitative RT-PCR, FACS analysis, and enzymatic assays, we show that miR221 is translocated via human TNBC-derived MV, which upon fusion with recipient cells, enhance their proliferation, survival, and metastasis both in vitro and in vivo by inducing the epithelial-to-mesenchymal transition (EMT).
T7 1343-1572 DRI_Background denotes Administration of anti-miR221 significantly impaired MV-induced expression of the mesenchymal markers Snail, Slug, N-cadherin, and vimentin in the recipient cells, whereas restoring expression of the epithelial marker E-cadherin.
T8 1573-1597 DRI_Outcome denotes We also demonstrate that
T9 1619-1763 DRI_Outcome denotes targets phosphatase and tensin homolog (PTEN) in the recipient cells, followed by AKT Ser/Thr kinase (AKT)/NF-κB activation, which promotes EMT.
T10 1764-1909 DRI_Background denotes Moreover, elevated miR221 levels in MV derived from human TNBC patients' blood could induce cell proliferation and metastasis in recipient cells.
T11 1910-2042 DRI_Approach denotes In summary, miR221 transfer from TNBC cells via PAR2-derived MV induces EMT and enhances the malignant potential of recipient cells.