PubMed_ArguminSci  

PubMed:30718974 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 124-216 DRI_Background denotes The intestinal epithelium is the first determining barrier to the drugs administered per os.
T2 444-576 DRI_Background denotes In this study, the effect of four drinking water supplements on CYP mRNA levels of porcine intestinal epithelial cells was examined.
T3 577-716 DRI_Background denotes Further goal of the study is to describe the effect of these feed additives on the proinflammatory response of the LPS-treated enterocytes.
T4 717-832 DRI_Background denotes The nontransformed porcine intestinal epithelial cells (IPEC-J2) were grown on six-well polyester membrane inserts.
T5 833-1049 DRI_Background denotes Cell cultures were treated with LPS (10 μg/ml), β-glucan (5 and 50 μg/ml), sanguinarine-containing additive (5 and 50 μg/ml), drinking water acidifier (0.1 and 1 μl/ml), and fulvic acid (25 and 250 μg/ml) for 1 hour.
T6 1050-1148 DRI_Approach denotes Cells were washed with culture medium and incubated for additional 1 h before total RNA isolation.
T7 1149-1229 DRI_Background denotes IL-6, IL-8, TNF-α, HSP70, CYP1A1, CYP1A2, and CYP3A29 mRNA levels were measured.
T8 1230-1298 DRI_Background denotes The LPS treatment upregulated the gene expression of IL-8 and TNF-α.
T9 1299-1438 DRI_Background denotes The relative gene expression of IL-6 remained unchanged and TNF-α and HSP70 were downregulated after the treatment with each feed additive.
T10 1439-1571 DRI_Background denotes CYP1A1 and CYP1A2 expressions increased after sanguinarine-containing solution, fulvic acid, and drinking water acidifier treatment.
T11 1572-1724 DRI_Outcome denotes None of the treatments changed the gene expression of CYP3A29, responsible for the metabolism of the majority of drug substances used in swine industry.
T12 1725-1943 DRI_Approach denotes The feed additive substances inhibited the expression of proinflammatory mediators HSP70 and TNF-α; however, β-glucan and fulvic acid elevated the production of the chemokine IL-8 mRNA in endotoxin-treated enterocytes.
T13 1944-2077 DRI_Approach denotes All acidic supplements increased the expression of CYP1A1 gene; their constituents may serve as a ligand of CYP1A1 nuclear receptors.