PubMed_ArguminSci  

PubMed:29195784 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 152-229 DRI_Approach denotes pheral antigen plays a crucial role in Echinococcus granulosus pathogenicity.
T2 230-418 DRI_Outcome denotes Considering the diversity of antigen among different EG95 isolates, it seems to be an ideal antigen for designing a universal multivalent minigene vaccine, so-called multi-epitope vaccine.
T3 419-576 DRI_Approach denotes This is the first in silico study to design a construct for the development of global EG95-based hydatid vaccine against E. granulosus in intermediate hosts.
T4 577-692 DRI_Approach denotes After antigen sequence selection, the three-dimensional structure of EG95 was modeled and multilaterally validated.
T5 693-876 DRI_Outcome denotes The preliminary parameters for B-cell epitope prediction were implemented such as the possible transmembrane helix, signal peptide, post-translational modifications and allergenicity.
T6 877-990 DRI_Background denotes The high ranked linear and conformational B-cell epitopes derived from several online web-servers (e.g., ElliPro,
T7 1004-1049 DRI_Background denotes , BcePred, ABCpred, SVMTrip, IEDB algorithms,
T8 1061-1064 DRI_Background denotes and
T9 1079-1174 DRI_Background denotes ) were utilized for multiple sequence alignment and then for engineering the vaccine construct.
T10 1175-1345 DRI_Approach denotes T-helper based epitopes were predicted by molecular docking between the high frequent ovar class II allele (Ovar-DRB1*1202) and hexadecamer fragments of the EG95 protein.
T11 1346-1411 DRI_Approach denotes Having used the immune-informatics tools, we formulated the first
T12 1432-1516 DRI_Approach denotes vaccine based on T-helper epitope with high-binding affinity to the ovar MHC allele.
T13 1517-1595 DRI_Approach denotes This designed construct was analyzed for different physicochemical properties.
T14 1596-1674 DRI_Background denotes It was also codon-optimized for high-level expression in Escherichia coli k12.
T15 1675-1893 DRI_Outcome denotes Taken all, we propose the present in silico vaccine constructs as a promising platform for the generation of broadly protective vaccines for species and genus-specific immunization of the natural hosts of the parasite.