| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
197-297 |
DRI_Background |
denotes |
Rabbit haemorrhagic disease virus (RHDV) is a calicivirus that infects and frequently kills rabbits. |
| T2 |
298-509 |
DRI_Outcome |
denotes |
Previously, we showed that the RHDV RNA-dependent RNA polymerase (RdRp) is associated with distinct, but yet uncharacterised subcellular structures and is capable of inducing a redistribution of Golgi membranes. |
| T3 |
510-635 |
DRI_Approach |
denotes |
In this study, we identified a partially hidden hydrophobic motif that determines the subcellular localisation of recombinant |
| T4 |
646-667 |
DRI_Approach |
denotes |
in transfected cells. |
| T5 |
668-813 |
DRI_Approach |
denotes |
This novel motif, 189LLWGCDVGVAVCAAAVFHNICY210, is located within the F homomorph, between the conserved F3 and A motifs of the core RdRp domain. |
| T6 |
814-1015 |
DRI_Background |
denotes |
Amino acid substitutions that decrease the hydrophobicity of this motif reduced the ability of the protein to accumulate in multiple subcellular foci and to induce a rearrangement of the Golgi network. |
| T7 |
1016-1088 |
DRI_Challenge |
denotes |
Furthermore, preliminary molecular dynamics simulations suggest that the |
| T8 |
1099-1234 |
DRI_Challenge |
denotes |
could align with the negatively charged surfaces of biological membranes and undergo a conformational change involving the F homomorph. |
| T9 |
1235-1378 |
DRI_Background |
denotes |
These changes would expose the newly identified hydrophobic motif so it could immerse itself into the outer leaflet of intracellular membranes. |
