PubMed_ArguminSci  

PubMed:27792889 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T1 85-210 DRI_Outcome denotes This study tested the hypothesis that salsolinol, a derivative of dopamine, affects GnRH and LH secretion in lactating sheep.
T2 211-451 DRI_Outcome denotes In the in vivo experiment, the structural analogue of salsolinol, 1-methyl-3,4-dihydroisoquinoline (1-MeDIQ), was infused into the infundibular nucleus-median eminence of sheep at the fifth wk of lactation to antagonize salsolinol's action.
T3 452-617 DRI_Background denotes Simultaneously, cerebrospinal fluid from the third brain ventricle, to determine GnRH concentration, and plasma samples, to measure LH concentration, were collected.
T4 618-814 DRI_Background denotes In the in vitro experiment, the anterior pituitary (AP) explants from weaned sheep were incubated in culture medium containing 2 doses of salsolinol, 20 and 100 μg/mL (S20 and S100, respectively).
T5 815-950 DRI_Background denotes The concentration of LH in the collected media and relative expression of LHβ subunit messenger RNA in the AP explants were determined.
T6 951-1203 DRI_Approach denotes No significant difference was found in mean GnRH concentration in response to 1-MeDIQ infusion, but both mean plasma LH concentration and LH pulse frequency increased significantly (P < 0.001 and P < 0.05, respectively) compared with those in controls.
T7 1204-1346 DRI_Approach denotes Significantly higher LH concentrations occurred during the first (P < 0.001), second (P < 0.001), and fourth (P < 0.05) h of 1-MeDIQ infusion.
T8 1347-1551 DRI_Approach denotes In the in vitro study, both the S20 and S100 doses of salsolinol caused a significant decrease in the mean medium LH concentration compared with that in the control (P < 0.01 and P < 0.001, respectively).
T9 1552-1654 DRI_Approach denotes Salsolinol had no effect on the relative LHβ subunit messenger RNA expression in the incubated tissue.
T10 1655-1802 DRI_Outcome denotes In conclusion, salsolinol is a potential inhibitor of the secretory activity of the gonadotropic axis in lactating sheep, at least at the AP level.
T11 1803-1977 DRI_Background denotes Although no significant changes in GnRH release were directly confirmed, an increase in the frequency of LH pulses does not allow to exclude the central action of salsolinol.