PubMed_ArguminSci  

PubMed:26122871 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 99-255 DRI_Background denotes Organ culture is an increasingly important tool in research, with advantages over monolayer cell culture due to the inherent natural environment of tissues.
T2 256-309 DRI_Background denotes Successful organ cultures must retain cell viability.
T3 310-503 DRI_Background denotes The aim of this study was to produce viable and non-viable osteochondral organ cultures, to assess the accumulation of soluble markers in the conditioned medium for predicting tissue viability.
T4 504-689 DRI_Outcome denotes Porcine femoral osteochondral plugs were cultured for 20 days, with the addition of Triton X-100 on day 6 (to induce necrosis), camptothecin (to induce apoptosis) or no toxic additives.
T5 690-791 DRI_Background denotes Tissue viability was assessed by the tissue destructive XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]
T6 836-916 DRI_Background denotes salt) assay method and LIVE/DEAD® staining of the cartilage at days 0, 6 and 20.
T7 917-1016 DRI_Background denotes Tissue structure was assessed by histological evaluation using haematoxylin & eosin and safranin O.
T8 1232-1367 DRI_Background denotes Necrotic cultures immediately showed a reduction in glucose consumption, and an immediate increase in LDH, GAG, MMP-2 and MMP-9 levels.
T9 1368-1570 DRI_Background denotes Apoptotic cultures showed a delayed reduction in glucose consumption and delayed increase in LDH, a small rise in MMP-2 and MMP-9, but no significant effect on GAGs released into the conditioned medium.
T10 1571-1744 DRI_Outcome denotes The data showed that tissue viability could be monitored by assessing the conditioned medium for the aforementioned markers, negating the need for tissue destructive assays.
T11 1745-2018 DRI_Background denotes Physiologically relevant whole- or part-joint organ culture models, necessary for research and pre-clinical assessment of therapies, could be monitored this way, reducing the need to sacrifice tissues to determine viability, and hence reducing the sample numbers necessary.