| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
137-325 |
DRI_Background |
denotes |
Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. |
| T2 |
326-497 |
DRI_Background |
denotes |
Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 μatm) and temperature (18°C). |
| T3 |
498-696 |
DRI_Background |
denotes |
Isolated perfused gill preparations were established to determine gill thermal plasticity during acute exposures (10-22°C) and in vivo costs of Na(+)/K(+)-ATPase activity, protein and RNA synthesis. |
| T4 |
697-822 |
DRI_Approach |
denotes |
Maximum enzyme capacities of F1Fo-ATPase, H(+)-ATPase and Na(+)/K(+)-ATPase were measured in vitro in crude gill homogenates. |
| T5 |
823-968 |
DRI_Challenge |
denotes |
After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. |
| T6 |
969-1061 |
DRI_Background |
denotes |
The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. |
| T7 |
1062-1219 |
DRI_Background |
denotes |
In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na(+)/K(+)-ATPase activity was reduced by 30% below rates of control fish. |
| T8 |
1220-1414 |
DRI_Approach |
denotes |
This contrasted in vitro capacities of Na(+)/K(+)-ATPase, which remained unchanged under elevated CO2 at 10°C, and earlier studies which had found a strong upregulation under severe hypercapnia. |
| T9 |
1656-1858 |
DRI_Challenge |
denotes |
We conclude that in vivo ion regulatory energy demand is lowered under moderately elevated CO2 levels despite the stronger thermal response of total gill respiration and the upregulation of F1Fo-ATPase. |
| T10 |
1859-1909 |
DRI_Approach |
denotes |
This effect is maintained at elevated temperature. |
