| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
138-308 |
DRI_Background |
denotes |
Bioactive compound-rich brown seaweeds are demonstrated to have numerous health benefits including anti-microbial and immunomodulatory bioactivities in the pig intestine. |
| T2 |
309-516 |
DRI_Background |
denotes |
In this study, the immunomodulating effects of extracts of brown seaweed (Ascophyllum nodosum and Fucus serratus) were evaluated on the porcine colon using a bacterial lipopolysaccharide (LPS) ex vivo model. |
| T3 |
517-724 |
DRI_Outcome |
denotes |
Approximately 1.5 × 1.5 cm of pig colon (n = 6) was stripped of its overlying muscle layer and incubated in 1 mL Dulbecco's Modified Eagle Medium containing bacterial LPS (10 μg) and seaweed extracts (1 mg). |
| T4 |
725-876 |
DRI_Background |
denotes |
Gene expression of interleukin-8 (IL-8) and interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNFA) were measured using quantitative real time PCR. |
| T5 |
877-1124 |
DRI_Background |
denotes |
In contrast to the low level of expression of IL-8, IL-6, and TNFA genes in the colonic tissue at 0 h, LPS treatment increased (P < 0.05) the expression of IL-8, IL-6, and TNFA genes to 2.38 ± 0.86, 1.90 ± 0.66, and 1.90 ± 0.57 fold, respectively. |
| T6 |
1125-1221 |
DRI_Approach |
denotes |
This pro-inflammatory response induced by the LPS was suppressed by the extracts of Ascophyllum. |
| T7 |
1222-1500 |
DRI_Outcome |
denotes |
Ascophyllum extract reduced (P < 0.05) the expression of IL-8, IL-6, and TNFA genes to 0.99 ± 0.53, 0.75 ± 0.33, and 1.01 ± 0.17 fold, and Fucus extract reduced (P < 0.05) the expression of the corresponding genes to 0.70 ± 0.32, 0.69 ± 0.38, and 1.15 ± 0.25 fold, respectively. |
| T8 |
1501-1672 |
DRI_Background |
denotes |
It is concluded that the extracts of Ascophyllum and Fucus seaweeds have potential to suppress the pro-inflammatory response induced by the bacterial LPS in the pig colon. |
