PubMed_ArguminSci  

PubMed:23365272 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 74-274 DRI_Background denotes Surgical removal of porcine intestinal tissue followed by an ex vivo challenge is an alternative technique of testing the anti-inflammatory effect of bioactive compounds in the intestine of live pigs.
T2 275-416 DRI_Outcome denotes We investigated the effects of ex vivo incubation of porcine colonic tissue on the quantity and quality of total RNA over a 12-h time period.
T3 417-593 DRI_Approach denotes Colonic tissue of pig (n = 6) was surgically removed immediately postslaughter and incubated for 0, 3, 6, and 12 h in a humidified cell culture incubator with 5% CO(2) at 37°C.
T4 594-643 DRI_Background denotes Tissue samples were processed for RNA extraction.
T5 644-774 DRI_Background denotes The quantity and quality of total RNA were assessed on a NanoDrop Spectrophotometer and an Agilent 2100 Bioanalyzer, respectively.
T6 775-878 DRI_Approach denotes Ex vivo incubation had an effect on both the quantity (P < 0.001) and quality (P < 0.001) of total RNA.
T7 879-1070 DRI_Approach denotes Relative to the RNA yield at 0 h (505.0 ± 48.64 μg/mg), the yield was significantly reduced after 6 h (227.6 ± 25.52 μg/mg; P < 0.001) and 12 h (159.3 ± 24.19 μg/mg; P < 0.001) of incubation.
T8 1071-1152 DRI_Approach denotes The 28S and 18S rRNA bands were visibly intact after 0, 3, and 6 h of incubation.
T9 1153-1223 DRI_Background denotes However, after 12 h of incubation, a degraded RNA profile was evident.
T10 1224-1396 DRI_Outcome denotes The RNA integrity number (RIN) values for the 0, 3, 6, and 12 h of incubation were 9.4 ± 0.10, 9.0 ± 0.10, 6.7 ± 0.17 (P < 0.001), and 3.3 ± 0.24 (P < 0.001), respectively.
T11 1397-1640 DRI_Approach denotes The transcript abundances of 4 constitutively expressed genes glyceraldehyde 3-phosphate dehydrogenase (GAPDH), beta actin (ACTB), beta 2-microglobulin (B2M), and peptidylprolyl isomerase A (PPIA) were reduced at both 6 and 12 h of incubation.
T12 1641-1798 DRI_Background denotes It is concluded that ex vivo incubation of porcine colonic tissue up to 3 h postmortem generates good quality total RNA suitable for gene expression studies.