| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
145-308 |
DRI_Background |
denotes |
Both elevated iron concentrations and the resulting oxidative stress condition are common signs in retinas of patients with age-related macular degeneration (AMD). |
| T2 |
309-403 |
DRI_Background |
denotes |
The role of phospholipase A(2) (PLA(2)) during iron-induced retinal toxicity was investigated. |
| T3 |
681-875 |
DRI_Outcome |
denotes |
Incubation with Fe(2+) led to a time- and concentration-dependent increase in retinal lipid peroxidation levels whereas retinal cell viability was only affected after 60 min of oxidative injury. |
| T4 |
876-1105 |
DRI_Background |
denotes |
A differential release of arachidonic acid (AA) and palmitic acid (PAL) catalyzed by cPLA(2) and iPLA(2) activities, respectively, was also observed in microsomal and cytosolic fractions obtained from retinas incubated with iron. |
| T5 |
1106-1220 |
DRI_Background |
denotes |
AA release diminished as the association of cyclooxygenase-2 increased in microsomes from retinas exposed to iron. |
| T6 |
1221-1433 |
DRI_Background |
denotes |
Retinal lipid peroxidation and cell viability were also analyzed in the presence of cPLA(2) inhibitor, arachidonoyl trifluoromethyl ketone (ATK), and in the presence of iPLA(2) inhibitor, bromoenol lactone (BEL). |
| T7 |
1434-1534 |
DRI_Background |
denotes |
ATK decreased lipid peroxidation levels and also ERK1/2 activation without affecting cell viability. |
| T8 |
1589-1766 |
DRI_Outcome |
denotes |
Our results demonstrate that iPLA(2) and cPLA(2) are differentially regulated and that they selectively participate in retinal signaling in an experimental model resembling AMD. |
