PubMed_ArguminSci  

PubMed:22079246 JSONTXT 44 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 93-196 DRI_Background denotes Human cell line secretome represents a valuable source of therapeutic targets and candidate biomarkers.
T2 197-289 DRI_Background denotes Secreted proteins found in biological fluids or culture media are by essence highly diluted.
T3 290-432 DRI_Background denotes Secretome investigation with proteomic approaches is hardly compatible with the high content of proteins found in complete cell culture media.
T4 433-516 DRI_Background denotes Therefore, many studies are currently done with media containing few or no protein.
T5 517-579 DRI_Approach denotes Such conditions may perturb cell metabolism and proliferation.
T6 580-701 DRI_Outcome denotes Here, we compared seventeen different compositions of culture media for the human bronchial epithelial BEAS-2B cell line.
T7 702-793 DRI_Background denotes Cell viability, proliferation rate and initial protein charge were systematically compared.
T8 794-908 DRI_Outcome denotes We have shown that an important difficulty for the proteomic analysis is due to the presence of detergents such as
T9 923-963 DRI_Outcome denotes which hinders peptide mass spectrometry.
T10 964-1096 DRI_Background denotes The high glucose containing DMEM medium which is free of proteins was shown to preserve a good viability and proliferation of cells.
T11 1097-1202 DRI_Approach denotes With this conditioning medium, we identified 81 extracellular proteins in the secretome of BEAS-2B cells.
T12 1203-1355 DRI_Approach denotes Moreover, to illustrate this approach, we exposed BEAS-2B cells to a low toxic dose of CoCl(2,) and found 24 extracellular proteins modulated by cobalt.