PubMed_ArguminSci  

PubMed:20573980 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 89-168 DRI_Background denotes Dynamic regulation of cytoskeletal contractility through phosphorylation of the
T2 189-290 DRI_Background denotes regulatory light chain (MRLC) provides an essential source of tension for shaping epithelial tissues.
T3 291-457 DRI_Challenge denotes Rho GTPase and its effector kinase ROCK have been implicated in regulating MRLC phosphorylation in vivo, but evidence suggests that other mechanisms must be involved.
T4 458-632 DRI_Outcome denotes Here, we report the identification of a single Drosophila homologue of the Death-associated protein kinase (DAPK) family, called Drak, as a regulator of MRLC phosphorylation.
T5 633-765 DRI_Approach denotes Based on analysis of null mutants, we find that Drak broadly promotes proper morphogenesis of epithelial tissues during development.
T6 766-910 DRI_Background denotes Drak activity is largely redundant with that of the Drosophila ROCK orthologue, Rok, such that it is essential only when Rok levels are reduced.
T7 911-1030 DRI_Outcome denotes We demonstrate that these two kinases synergistically promote phosphorylation of Spaghetti squash (Sqh), the Drosophila
T8 1046-1056 DRI_Outcome denotes , in vivo.
T9 1057-1206 DRI_Background denotes The lethality of drak/rok mutants can be rescued by restoring Sqh activity, indicating that Sqh is the critical common effector of these two kinases.
T10 1207-1400 DRI_Background denotes These results provide the first evidence that DAPK family kinases regulate actin dynamics in vivo and identify Drak as a novel component of the signaling networks that shape epithelial tissues.