| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
138-249 |
DRI_Background |
denotes |
Ex vivo culture of arteries and veins is an established tool for investigating mechanically induced remodeling. |
| T2 |
250-334 |
DRI_Background |
denotes |
Porcine saphenous veins (PSV) cultured ex vivo with a venous mechanical environment, |
| T3 |
367-669 |
DRI_Background |
denotes |
medium and standard cell-culture conditions (5% CO₂ and 95% balance air ~140 mmHg pO₂) develop intimal hyperplasia (IH), increased cellular proliferation, decreased compliance and exhibit inward eutrophic remodeling thereby suggesting that nonmechanical factors stimulate some changes observed ex vivo. |
| T4 |
670-789 |
DRI_Outcome |
denotes |
Herein we explore the contribution of exposure to greater than venous pO₂ and serum to these changes in cultured veins. |
| T5 |
790-930 |
DRI_Background |
denotes |
Removing serum from culture medium did not inhibit development of IH, but did reduce cellular proliferation and inward eutrophic remodeling. |
| T6 |
931-1005 |
DRI_Background |
denotes |
In contrast, veins perfused using reduced pO₂ (75 mmHg) showed reduced IH. |
| T7 |
1006-1223 |
DRI_Outcome |
denotes |
Among the statically cultured vessels, veins cultured at arterial pO₂ (95 mmHg) and above showed IH as well as increase in proliferation and vessel weight compared to fresh veins; veins cultured at venous pO₂ did not. |
| T8 |
1224-1472 |
DRI_Approach |
denotes |
Taken together, these data suggest that exposure of SV to arterial pO₂ stimulates IH and cellular proliferation independent of changes in the mechanical environment, which might provide insight into the etiology of IH in SV used as arterial grafts. |
