PubMed_ArguminSci  

PubMed:18182054 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 97-333 DRI_Challenge denotes Neuritic retraction represents a prominent feature of the degenerative phenotype associated with mutations in leucine rich repeat kinase 2 (LRRK2) that are implicated in autosomal dominant and some cases of sporadic Parkinson's disease.
T2 334-461 DRI_Background denotes Alterations in macroautophagy, the vacuolar catabolism of cytoplasmic constituents, have been described in Parkinson's disease.
T3 462-623 DRI_Approach denotes In this study, we utilized retinoic-acid differentiated SH-SY5Y cells to determine whether autophagy contributes to mutant LRRK2-associated neurite degeneration.
T4 624-639 DRI_Background denotes Transfection of
T5 667-852 DRI_Background denotes cells with LRRK2 cDNA containing the common G2019S mutation resulted in significant decreases in neurite length, which were not observed in cells transfected with wild type LRRK2 or its
T6 872-881 DRI_Background denotes mutation.
T7 882-888 REPLACED denotes G2019S
T8 895-1213 DRI_Outcome denotes transfected cells also exhibited striking increases in autophagic vacuoles in both neuritic and somatic compartments, as demonstrated by fluorescence and western blot analysis of the autophagy marker green fluorescent protein-tagged microtubule-associated protein Light Chain 3 and by transmission electron microscopy.
T9 1214-1343 DRI_Background denotes RNA interference knockdown of LC3 or Atg7, two essential components of the conserved autophagy machinery, reversed the effects of
T10 1357-1440 DRI_Background denotes expression on neuronal process length, whereas rapamycin potentiated these effects.
T11 1441-1633 DRI_Background denotes The mitogen activated protein kinase/extracellular signal regulated protein kinase (MAPK/ERK) kinase (MEK) inhibitor 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126) reduced
T12 1657-1730 DRI_Background denotes autophagy and neurite shortening, implicating MAPK/ERK-related signaling.
T13 1731-1845 DRI_Background denotes These results indicate an active role for autophagy in neurite remodeling induced by pathogenic mutation of LRRK2.