PubMed_ArguminSci  

PubMed:17389652 JSONTXT 53 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T1 143-268 DRI_Outcome denotes The receptor specificity of influenza viruses is one factor that allows avian influenza viruses to cross the species barrier.
T2 269-481 DRI_Outcome denotes The recent transmissions of avian H5N1 and H9N2 influenza viruses from chickens and/or quails to humans indicate that avian influenza viruses can directly infect humans without an intermediate host, such as pigs.
T3 482-727 DRI_Approach denotes In this study, we used two strains of influenza A virus (A/PR/8/34, which preferentially binds to an avian-type receptor, and A/Memphis/1/71, which preferentially binds to a human-type receptor) to probe the receptor specificities in host cells.
T4 728-837 DRI_Approach denotes Epithelial cells of both quail and chicken intestines (colons) could bind both avian- and human-type viruses.
T5 838-970 DRI_Background denotes Infected cultured quail colon cells expressed viral protein and allowed replication of the virus strain A/PR/8/34 or A/Memphis/1/71.
T6 971-1184 DRI_Challenge denotes To understand the molecular basis of these phenomena, we further investigated the abundance of sialic acid (Sia) linked to galactose (Gal) by the alpha2-3 linkage (Siaalpha2-3Gal) and Siaalpha2-6Gal in host cells.
T7 1185-1308 DRI_Outcome denotes In glycoprotein and glycolipid fractions from quail and chicken colon epithelial cells, there were some bound components of
T8 1334-1558 DRI_Outcome denotes lectins, Maackia amurensis agglutinin (specific for Siaalpha2-3 Gal) and Sambucus nigra agglutinin (specific for Siaalpha2-6Gal), indicating that both Siaalpha2-3Gal and Siaalpha2-6Gal exist in quail and chicken colon cells.
T9 1559-1877 DRI_Approach denotes Furthermore, we demonstrated by fluorescence high-performance liquid chromatography (HPLC) analysis that 5-N-acetylneuraminic acid was the main molecular species of Sia, and we demonstrated by multi-dimensional HPLC mapping and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis that
T10 1904-1911 DRI_Approach denotes glycans
T11 1932-1988 DRI_Approach denotes at the terminal Gal residue(s) are major (more than 79%)
T12 2006-2079 DRI_Approach denotes expressed by intestinal epithelial tissues in both the chicken and quail.
T13 2080-2322 DRI_Outcome denotes Taken together, these results indicate that quails and chickens have molecular characterization as potential intermediate hosts for avian influenza virus transmission to humans and could generate new influenza viruses with pandemic potential.