PubMed_ArguminSci  

PubMed:12076079 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 122-338 DRI_Approach denotes Matrix metalloproteinases (MMPs) are endopeptidases that degrade the extracellular matrix (ECM) and are involved in the pathogenesis of retinal degeneration along with tissue inhibitors of metalloproteinases (TIMPs).
T2 339-550 DRI_Background denotes The present study examined the expression and activation of two specific members of MMPs (MMP-2 and MMP-9) and their related inhibitors (TIMP-1 and TIMP-2) in an experimental retinal ischemia-reperfusion injury.
T3 551-643 DRI_Background denotes Retinal ischemia-reperfusion injury (RIRI) was induced in adult rats with a ligation method.
T4 644-763 DRI_Background denotes After one hour of ischemia and a varied reperfusion time (0, 3, 6, 12, 24, 48 and 76 hr), the rat eyes were enucleated.
T5 764-847 DRI_Background denotes Retinal extracts underwent zymographic analysis to measure the activity of MMP-2/9.
T6 848-917 DRI_Background denotes The activity of TIMP-1 and TIMP-2 was measured by reverse zymography.
T7 918-965 DRI_Background denotes The protein level was examined by Western blot.
T8 966-1083 DRI_Background denotes Immunohistochemistry analysis was undertaken to assess the anatomical distribution of MMP-9 in the retina after RIRI.
T9 1084-1174 DRI_Background denotes The gelatinolytic activity of ProMMP-2 (72 kDa) was increased markedly at 6 hr after RIRI.
T10 1175-1338 DRI_Approach denotes ProMMP-9 (92 kDa) was not detected in the control specimens, while it appeared at 3 hr, increased markedly at 6 hr, and reached maximal levels at 24 hr after RIRI.
T11 1339-1602 DRI_Background denotes The gelatinolytic activity found ian retinal extracts was shown to be inhibited by 10 m M EDTA and activated in vitro by a known metalloproteinase activator (4-aminophenylmercuric acetate (APMA)), indicating that these enzymes were of the metalloproteinase class.
T12 1603-1694 DRI_Background denotes By western blot, MMP-2/9 levels increased parallel to protein activity level in zymography.
T13 1695-1829 DRI_Background denotes No corresponding increase in TIMP-1 and TIMP-2 protein activity and protein level was detected by reverse zymography and western blot.
T14 1830-1925 DRI_Background denotes Elevated levels of MMP-9 and its distribution in retina were confirmed by immunohistochemistry.
T15 1926-2057 DRI_Background denotes Expression of MMP-9 was detected in the inner and outer segments of rat retina, and the level becomes stronger at 24 hr after RIRI.
T16 2058-2248 DRI_Background denotes In this study, ProMMP-2 and ProMMP-9 were expressed and increased significantly, but their inhibitors (TIMP-1 and TIMP-2) remained relatively unaltered in ischemic retina after RIRI in rats.
T17 2249-2368 DRI_Challenge denotes These results suggest that MMP-2 and MMP-9 may play an important role in the pathomechanism of retinal ischemic injury.