| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-100 |
Sentence |
denotes |
PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8. |
| T2 |
101-214 |
Sentence |
denotes |
Many eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). |
| T3 |
215-380 |
Sentence |
denotes |
The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. |
| T4 |
381-494 |
Sentence |
denotes |
During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. |
| T5 |
495-572 |
Sentence |
denotes |
It was known that the GPI transamidase is a complex containing GAA1 and GPI8. |
| T6 |
573-640 |
Sentence |
denotes |
Here, we report two new components of this enzyme: PIG-S and PIG-T. |
| T7 |
641-752 |
Sentence |
denotes |
To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. |
| T8 |
753-887 |
Sentence |
denotes |
PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. |
| T9 |
888-1056 |
Sentence |
denotes |
We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. |
| T10 |
1057-1169 |
Sentence |
denotes |
Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively. |
