| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-132 |
Sentence |
denotes |
A recurrent general RNA binding domain appended to plant methionyl-tRNA synthetase acts as a cis-acting cofactor for aminoacylation. |
| T2 |
133-195 |
Sentence |
denotes |
The cDNA encoding rice methionyl-tRNA synthetase was isolated. |
| T3 |
196-280 |
Sentence |
denotes |
The protein exhibited a C-terminal polypeptide appended to a classical MetRS domain. |
| T4 |
281-475 |
Sentence |
denotes |
This supplementary domain is related to endothelial monocyte activating polypeptide II (EMAPII), a cytokine produced in mammals after cleavage of p43, a component of the multisynthetase complex. |
| T5 |
476-543 |
Sentence |
denotes |
It is also related to Arc1p and Trbp111, two tRNA binding proteins. |
| T6 |
544-627 |
Sentence |
denotes |
We expressed rice MetRS and a derivative with a deletion of its EMAPII-like domain. |
| T7 |
628-735 |
Sentence |
denotes |
Band-shift analysis showed that this extra-domain provides MetRS with non-specific tRNA binding properties. |
| T8 |
736-921 |
Sentence |
denotes |
The EMAPII-like domain contributed a 10-fold decrease in K:(M) for tRNA in the aminoacylation reaction catalyzed by the native enzyme, as compared with the C-terminally truncated MetRS. |
| T9 |
922-1054 |
Sentence |
denotes |
Consequently, the EMAPII domain provides MetRS with a better catalytic efficiency at the free tRNA concentration prevailing in vivo. |
| T10 |
1055-1144 |
Sentence |
denotes |
This domain binds the acceptor minihelix of tRNA(Met) and facilitates its aminoacylation. |
| T11 |
1145-1366 |
Sentence |
denotes |
These results suggest that the EMAPII module could be a relic of an ancient tRNA binding domain that was incorporated into primordial synthetases for aminoacylation of RNA minihelices taken as the ancestor of modern tRNA. |
