| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-101 |
Sentence |
denotes |
Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2. |
| T2 |
102-222 |
Sentence |
denotes |
Glycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors. |
| T3 |
223-396 |
Sentence |
denotes |
Biosynthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), which transfers N-acetylglucosamine from UDP- N-acetylglucosamine to phosphatidylinositol. |
| T4 |
397-515 |
Sentence |
denotes |
GPI-GnT is a uniquely complex glycosyltransferase, consisting of at least four proteins, PIG-A, PIG-H, PIG-C and GPI1. |
| T5 |
516-678 |
Sentence |
denotes |
Here, we report that GPI-GnT requires another component, termed PIG-P, and that DPM2, which regulates dolichol-phosphate-mannose synthase, also regulates GPI-GnT. |
| T6 |
679-774 |
Sentence |
denotes |
PIG-P, a 134-amino acid protein having two hydrophobic domains, associates with PIG-A and GPI1. |
| T7 |
775-856 |
Sentence |
denotes |
PIG-P is essential for GPI-GnT since a cell lacking PIG-P is GPI-anchor negative. |
| T8 |
857-1004 |
Sentence |
denotes |
DPM2, but not two other components of dolichol-phosphate-mannose synthase, associates with GPI-GnT through interactions with PIG-A, PIG-C and GPI1. |
| T9 |
1005-1200 |
Sentence |
denotes |
Lec15 cell, a null mutant of DPM2, synthesizes early GPI intermediates, indicating that DPM2 is not essential for GPI-GnT; however, the enzyme activity is enhanced 3-fold in the presence of DPM2. |
| T10 |
1201-1392 |
Sentence |
denotes |
These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI. |
