| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-114 |
Sentence |
denotes |
Reverse transcriptase-polymerase chain reaction validation of 25 "orphan" genes from Escherichia coli K-12 MG1655. |
| T2 |
115-479 |
Sentence |
denotes |
Despite the accumulation of sequence information sampling from a broad spectrum of phyla, newly sequenced genomes continue to reveal a high proportion (50%-30%) of "uncharacterized" genes, including a significant number of strictly "orphan" genes, i.e., putative open reading frames (ORFs) without any resemblance to previously determined protein-coding sequences. |
| T3 |
480-600 |
Sentence |
denotes |
Most genes found in databases have only been predicted by computer methods and have never been experimentally validated. |
| T4 |
601-769 |
Sentence |
denotes |
Although theoretical evolutionary arguments support the reality of genes when homologs are found in a variety of distant species, this is not the case for orphan genes. |
| T5 |
770-898 |
Sentence |
denotes |
Here, we report the direct reverse transcriptase-polymerase chain reaction assay of 25 strictly orphan ORFs of Escherichia coli. |
| T6 |
899-969 |
Sentence |
denotes |
Two growth conditions, exponential and stationary phases, were tested. |
| T7 |
970-1106 |
Sentence |
denotes |
Transcripts were identified for a total of 19 orphan genes, with 2 genes found to be expressed in only one of the two growth conditions. |
| T8 |
1107-1232 |
Sentence |
denotes |
Our results suggest that a vast majority of E. coli ORFs presently annotated as "hypothetical" correspond to bona fide genes. |
| T9 |
1233-1381 |
Sentence |
denotes |
By extension, this implies that randomly occurring "junk" ORFs have been actively counter selected during the evolution of the dense E. coli genome. |
