| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-90 |
Sentence |
denotes |
Human dolichol-phosphate-mannose synthase consists of three subunits, DPM1, DPM2 and DPM3. |
| T2 |
91-238 |
Sentence |
denotes |
Dolichol-phosphate-mannose (DPM) synthase generates mannosyl donors for glycosylphosphatidylinositols, N-glycan and protein O- and C-mannosylation. |
| T3 |
239-299 |
Sentence |
denotes |
In Saccharomyces cerevisiae, this enzyme is encoded by DPM1. |
| T4 |
300-488 |
Sentence |
denotes |
We reported previously that mammalian DPM synthase contains catalytic DPM1 and regulatory DPM2 subunits, and that DPM1 requires DPM2 for its stable expression in the endoplasmic reticulum. |
| T5 |
489-555 |
Sentence |
denotes |
Here we report that human DPM synthase consists of three subunits. |
| T6 |
556-694 |
Sentence |
denotes |
The third subunit, DPM3, comprises 92 amino acids associated with DPM1 via its C-terminal domain and with DPM2 via its N-terminal portion. |
| T7 |
695-741 |
Sentence |
denotes |
The stability of DPM3 was dependent upon DPM2. |
| T8 |
742-918 |
Sentence |
denotes |
However, overexpression of DPM3 in Lec15 cells, a null mutant of DPM2, restored the biosynthesis of DPM with an increase in DPM1, indicating that DPM3 directly stabilized DPM1. |
| T9 |
919-976 |
Sentence |
denotes |
Therefore, DPM2 stabilizes DPM3 and DPM3 stabilizes DPM1. |
| T10 |
977-1109 |
Sentence |
denotes |
DPM synthase activity was 10 times higher in the presence of DPM2, indicating that DPM2 also plays a role in the enzymatic reaction. |
| T11 |
1110-1297 |
Sentence |
denotes |
Schizosaccharomyces pombe has proteins that resemble three human subunits; S.pombe DPM3 restored biosynthesis of DPM in Lec15 cells, indicating its orthologous relationship to human DPM3. |
