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PubMed:10485883 JSONTXT 2 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-118 Sentence denotes Efficient adaptational demethylation of chemoreceptors requires the same enzyme-docking site as efficient methylation.
T2 119-367 Sentence denotes The mechanistic basis of sensory adaptation and gradient sensing in bacterial chemotaxis is reversible covalent modification of transmembrane chemoreceptors, methylation, and demethylation at specific glutamyl residues in their cytoplasmic domains.
T3 368-472 Sentence denotes These reactions are catalyzed by a dedicated methyltransferase CheR and a dedicated methylesterase CheB.
T4 473-593 Sentence denotes The esterase is also a deamidase that creates certain methyl-accepting glutamyls by hydrolysis of glutamine side chains.
T5 594-787 Sentence denotes We investigated the action of CheB and its activated form, phospho-CheB, on a truncated form of the aspartate receptor of Escherichia coli that was missing the last 5 aa of the intact receptor.
T6 788-884 Sentence denotes The deleted pentapeptide is conserved in several chemoreceptors in enteric and related bacteria.
T7 885-1065 Sentence denotes The truncated receptor was much less efficiently demethylated and deamidated than intact receptor, but essentially was unperturbed for kinase activation or transmembrane signaling.
T8 1066-1353 Sentence denotes CheB bound specifically to an affinity column carrying the isolated pentapeptide, implying that in the intact receptor the pentapeptide serves as a docking site for the methylesterase/deamidase and that the truncated receptor was inefficiently modified because the enzyme could not dock.
T9 1354-1555 Sentence denotes It is striking that the same pentapeptide serves as an activity-enhancing docking site for the methyltransferase CheR, the other enzyme involved in adaptational covalent modification of chemoreceptors.
T10 1556-1738 Sentence denotes A shared docking site raises the tantalizing possibility that relative rates of methylation and demethylation could be influenced by competition between the two enzymes at that site.