Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-104 |
Sentence |
denotes |
Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein. |
T2 |
105-178 |
Sentence |
denotes |
A rice Dwarf 1 gene was identified by using a map-based cloning strategy. |
T3 |
179-233 |
Sentence |
denotes |
Its recessive mutant allele confers a dwarf phenotype. |
T4 |
234-366 |
Sentence |
denotes |
Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3,185 d1 segregants. |
T5 |
367-483 |
Sentence |
denotes |
Southern hybridization analysis with this cDNA as a probe showed different band patterns in several d1 mutant lines. |
T6 |
484-592 |
Sentence |
denotes |
In at least four independent d1 mutants, no gene transcript was observed by Northern hybridization analysis. |
T7 |
593-751 |
Sentence |
denotes |
Sequencing analysis revealed that an 833-bp deletion had occurred in one of the mutant alleles, which resulted in an inability to express GTP-binding protein. |
T8 |
752-835 |
Sentence |
denotes |
A transgenic d1 mutant with GTP-binding protein gene restored the normal phenotype. |
T9 |
836-980 |
Sentence |
denotes |
We conclude that the rice Dwarf 1 gene encodes GTP-binding protein and that the protein plays an important role in plant growth and development. |
T10 |
981-1142 |
Sentence |
denotes |
Because the d1 mutant is classified as gibberellin-insensitive, we suggest that the GTP-binding protein might be associated with gibberellin signal transduction. |