| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-111 |
Sentence |
denotes |
Estrogen decreases TNF gene expression by blocking JNK activity and the resulting production of c-Jun and JunD. |
| T2 |
112-264 |
Sentence |
denotes |
Central to the bone-sparing effect of estrogen (E(2)) is its ability to block the monocytic production of the osteoclastogenic cytokine TNF-alpha (TNF). |
| T3 |
265-352 |
Sentence |
denotes |
However, the mechanism by which E(2) downregulates TNF production is presently unknown. |
| T4 |
353-540 |
Sentence |
denotes |
Transient transfection studies in HeLa cells, an E(2) receptor-negative line, suggest that E(2) inhibits TNF gene expression through an effect mediated by estrogen receptor beta (ERbeta). |
| T5 |
541-752 |
Sentence |
denotes |
We also report that in RAW 264.7 cells, an E(2) receptor-positive murine monocytic line, E(2) downregulates cytokine-induced TNF gene expression by decreasing the activity of the Jun NH(2)-terminal kinase (JNK). |
| T6 |
753-995 |
Sentence |
denotes |
The resulting diminished phosphorylation of c-Jun and JunD at their NH(2)-termini decreases the ability of these nuclear proteins to autostimulate the expression of the c-Jun and JunD genes, thus leading to lower production of c-Jun and JunD. |
| T7 |
996-1238 |
Sentence |
denotes |
The consequent decrease in the nuclear levels of c-Jun and JunD leads to diminished binding of c-Jun/c-Fos and JunD/c-Fos heterodimers to the AP-1 consensus sequence in the TNF promoter and, thus, to decreased transactivation of the TNF gene. |
