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PubMed:10366600 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-109 Sentence denotes Monocyte adhesion and spreading on human endothelial cells is dependent on Rho-regulated receptor clustering.
T2 110-221 Sentence denotes The GTPase Rho is known to mediate the assembly of integrin-containing focal adhesions and actin stress fibers.
T3 222-382 Sentence denotes Here, we investigate the role of Rho in regulating the distribution of the monocyte-binding receptors E-selectin, ICAM-1, and VCAM-1 in human endothelial cells.
T4 383-570 Sentence denotes Inhibition of Rho activity with C3 transferase or N19RhoA, a dominant negative RhoA mutant, reduced the adhesion of monocytes to activated endothelial cells and inhibited their spreading.
T5 571-661 Sentence denotes Similar effects were observed after pretreatment of endothelial cells with cytochalasin D.
T6 662-762 Sentence denotes In contrast, dominant negative Rac and Cdc42 proteins did not affect monocyte adhesion or spreading.
T7 763-1023 Sentence denotes C3 transferase and cytochalasin D did not alter the expression levels of monocyte-binding receptors on endothelial cells, but did inhibit clustering of E-selectin, ICAM-1, and VCAM-1 on the cell surface induced by monocyte adhesion or cross-linking antibodies.
T8 1024-1073 Sentence denotes Similarly, N19RhoA inhibited receptor clustering.
T9 1074-1257 Sentence denotes Monocyte adhesion and receptor cross-linking induced stress fiber assembly, and inhibitors of myosin light chain kinase prevented this response but did not affect receptor clustering.
T10 1258-1333 Sentence denotes Finally, receptor clusters colocalized with ezrin/moesin/ radixin proteins.
T11 1334-1588 Sentence denotes These results suggest that Rho is required in endothelial cells for the assembly of stable adhesions with monocytes via the clustering of monocyte-binding receptors and their association with the actin cytoskeleton, independent of stress fiber formation.