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PubMed:10362838 JSONTXT 61 Projects

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Id Subject Object Predicate Lexical cue
T1 0-162 Sentence denotes Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA.
T2 163-355 Sentence denotes Brefeldin A reversibly disassembles the Golgi complex, causing mixing of the Golgi cisternae with the ER while the trans Golgi network persists as part of a separate endosomal membrane system.
T3 356-513 Sentence denotes Because of this compartmental separation, Brefeldin A treatment has been used to map the sub-Golgi locations of several Golgi enzymes including GM2 synthase.
T4 514-750 Sentence denotes We previously proposed that GM2 synthase might be located in a distal portion of the Golgi complex which in the presence of Brefeldin A would be separated from the substrate ganglioside GM3 present in the mixed ER-Golgi membrane system.
T5 751-976 Sentence denotes In the present study we show using GM2 synthase chimeras that GM2 synthesis was blocked by Brefeldin A when GM2 synthase was distributed throughout all Golgi subcompartments or even when it was restricted to the medial Golgi.
T6 977-1164 Sentence denotes Because these findings opposed our speculation regarding a distal location of this enzyme, we sought an alternative explanation for the inhibition of ganglioside synthesis by Brefeldin A.
T7 1165-1279 Sentence denotes However, Brefeldin A did not degrade GM2 synthase, prevent its homodimerization, or inhibit its in vitro activity.
T8 1280-1444 Sentence denotes Brefeldin A did result in the conversion of a portion of membrane bound GM2 synthase into a soluble form which has minimal capability to produce GM2 in whole cells.
T9 1445-1587 Sentence denotes However, this conversion was not sufficient to explain the nearly total loss of GM2 production in intact cells in the presence of Brefeldin A.
T10 1588-1752 Sentence denotes Nevertheless, the results of this study indicate that Brefeldin A-induced inhibition of ganglioside synthesis cannot be used to deduce the location of GM2 synthase.