| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-125 |
Sentence |
denotes |
IncC of broad-host-range plasmid RK2 modulates KorB transcriptional repressor activity In vivo and operator binding in vitro. |
| T2 |
126-309 |
Sentence |
denotes |
The korAB operon of broad-host-range plasmid RK2 encodes five genes, two of which, incC and korB, belong to the parA and parB families, respectively, of genome partitioning functions. |
| T3 |
310-462 |
Sentence |
denotes |
Both korB and a third gene, korA, are responsible for coordinate regulation of operons encoding replication, transfer, and stable inheritance functions. |
| T4 |
463-525 |
Sentence |
denotes |
Overexpression of incC alone caused rapid displacement of RK2. |
| T5 |
526-611 |
Sentence |
denotes |
Using two different reporter systems, we show that incC modulates the action of KorB. |
| T6 |
612-732 |
Sentence |
denotes |
Using promoter fusions to the reporter gene xylE, we show that incC potentiates the repression of transcription by korB. |
| T7 |
733-969 |
Sentence |
denotes |
This modulation of korB activity was only observed with incC1, which encodes the full-length IncC (364 amino acids [aa]), whereas no effect was observed with incC2, which encodes a polypeptide of 259 aa that lacks the N-terminal 105 aa. |
| T8 |
970-1189 |
Sentence |
denotes |
Using bacterial extracts with IncC1 and IncC2 or IncC1 purified through the use of a His6 tail and Ni-agarose chromatography, we showed that IncC1 potentiates the binding of KorB to DNA at representative KorB operators. |
| T9 |
1190-1385 |
Sentence |
denotes |
The ability of IncC to stabilize KorB-DNA complexes suggests that these two proteins work together in the global regulation of many operons on the IncP-1 genomes, as well in plasmid partitioning. |
