| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-163 |
Sentence |
denotes |
Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis. |
| T2 |
164-269 |
Sentence |
denotes |
Glycosylphosphatidylinositol (GPI) is used as a membrane anchor by many eukaryotic cell-surface proteins. |
| T3 |
270-382 |
Sentence |
denotes |
The second step of GPI biosynthesis is de-N-acetylation of N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI). |
| T4 |
383-532 |
Sentence |
denotes |
We have previously cloned the rat PIG-L gene by expression cloning that complemented a mutant Chinese hamster ovary cell line defective in this step. |
| T5 |
533-683 |
Sentence |
denotes |
Here we show that recombinant rat PIG-L protein purified from Escherichia coli as a complex with GroEL has GlcNAc-PI de-N-acetylase activity in vitro. |
| T6 |
684-805 |
Sentence |
denotes |
The activity was not enhanced by GTP, which is known to enhance the de-N-acetylase activity of mammalian cell microsomes. |
| T7 |
806-946 |
Sentence |
denotes |
As with other de-N-acetylases that act on the GlcNAc moiety, metal ions, in particular Mn2+ and Ni2+, enhanced the enzyme activity of PIG-L. |
| T8 |
947-1081 |
Sentence |
denotes |
The Saccharomyces cerevisiae YMR281W open reading frame encodes a protein (termed Gpi12p) with 24% amino acid identity with rat PIG-L. |
| T9 |
1082-1254 |
Sentence |
denotes |
On transfection into mammalian PIG-L-deficient cells, this gene, GPI12, restored the cell-surface expression of GPI-anchored proteins and GlcNAc-PI de-N-acetylase activity. |
| T10 |
1255-1316 |
Sentence |
denotes |
The disruption of the gene caused lethality in S. cerevisiae. |
| T11 |
1317-1477 |
Sentence |
denotes |
These results indicate that GlcNAc-PI de-N-acetylase is conserved between mammals and yeasts and that the de-N-acetylation step is also indispensable in yeasts. |
