LitCovid-sentences  

PubMed:33177397 JSONTXT 19 Projects

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Id Subject Object Predicate Lexical cue
T1 0-92 Sentence denotes Establishing diagnostic algorithms for SARS-CoV-2 nucleic acid testing in clinical practice.
T2 93-104 Sentence denotes BACKGROUND:
T3 105-252 Sentence denotes Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic.
T4 253-393 Sentence denotes Our laboratory initially used a two-step molecular assay, first reported by Corman et al., for SARS-CoV-2 identification (the T-CDC method).
T5 394-730 Sentence denotes As rapid and accurate diagnosis of COVID-19 is required to control the spread of this infectious disease, the current study evaluated three commercially available assays, including the TaqPath COVID-19 Combo kit, the cobas® SARS-CoV-2 test and the Rendu 2019-nCoV Assay kit, to establish diagnostic algorithms for clinical laboratories.
T6 731-739 Sentence denotes METHODS:
T7 740-1027 Sentence denotes A total of 790 clinical specimens, including nasopharyngeal swabs, throat swabs, sputum, saliva, stool, endotracheal aspirate and serum were obtained from patients who were suspected or already confirmed to have COVID-19 at the Taipei Veterans General Hospital from February to May 2020.
T8 1028-1159 Sentence denotes These specimens were tested for SARS-CoV-2 using the different assays and the performance variance between the assays was analyzed.
T9 1160-1168 Sentence denotes RESULTS:
T10 1169-1399 Sentence denotes Of the assays we evaluated, the T-CDC method and the TaqPath COVID-19 Combo kit require lots of hands-on practical lab work, while the cobas® SARS-CoV-2 test and the Rendu 2019-nCoV Assay kit are fully automated detection systems.
T11 1400-1655 Sentence denotes The T-CDC method and the TaqPath COVID-19 Combo kit showed similar detection sensitivity, however, the T-CDC method frequently delivered false positive signals for E and/or RdRP gene detection, thus increasing the risk of reporting false positive results.
T12 1656-1867 Sentence denotes A manual test-based testing strategy combining the T-CDC method and the TaqPath COVID-19 Combo kit was developed, which demonstrated excellent concordance rates (>99%) with the cobas and Rendu automatic systems.
T13 1868-2023 Sentence denotes There were a few cases showing discrepant results, which may be due to the varied detection sensitivities as well as targets among the different platforms.
T14 2024-2099 Sentence denotes Moreover, the concordance rate between the cobas and Rendu assays was 100%.
T15 2100-2111 Sentence denotes CONCLUSION:
T16 2112-2265 Sentence denotes Based on our evaluation, two SARS-CoV-2 diagnostic algorithms, one focusing on the manual assays and the other on the automatic platforms, were proposed.
T17 2266-2507 Sentence denotes Our results provide valuable information that allow clinical laboratories to implement optimal diagnostic strategies for SARS-CoV-2 testing based on their clinical needs, such as test volume, turn-around time, and staff/resource limitations.