LitCovid-sentences  

PubMed:33075532 JSONTXT 20 Projects

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Id Subject Object Predicate Lexical cue
T1 0-126 Sentence denotes Higher binding affinity of Furin to SARS-CoV-2 spike (S) protein D614G could be associated with higher SARS-CoV-2 infectivity.
T2 127-137 Sentence denotes OBJECTIVE:
T3 138-252 Sentence denotes The coronavirus disease-19 (COVID-19) pandemic has caused an exponential rise in death rates and hospitalizations.
T4 253-375 Sentence denotes The aim of this study was to characterize the D614 G mutation of SARS-CoV-2 S-protein, which may affect viral infectivity.
T5 376-384 Sentence denotes METHODS:
T6 385-510 Sentence denotes The effect of D614 G mutation on the structure and thermodynamic stability of S-protein was analyzed using DynaMut and SCooP.
T7 511-640 Sentence denotes HDOCK and PRODIGY were used to model furin protease binding to the S-protein RARR cleavage site and calculate binding affinities.
T8 641-800 Sentence denotes Molecular dynamic (MD) simulations were used to predict S-protein apo structure, S-protein-furin complex structure, and the free binding energy of the complex.
T9 801-809 Sentence denotes RESULTS:
T10 810-966 Sentence denotes The D614 G mutation in the G clade of SARS-CoV-2 strains introduced structural mobility and decreased thermal stability of S-protein (ΔΔG: -0.086 kcal/mol).
T11 967-1084 Sentence denotes The mutation resulted in a stronger binding affinity (Kd = 1.6 × 10-8) to furin which may enhance S-protein cleavage.
T12 1085-1270 Sentence denotes Results were corroborated by MD simulations demonstrating higher binding energy of furin to S-protein D614 mutant (-61.9 kcal/mol compared with -56.78 kcal/mol for wild-type S-protein).
T13 1271-1283 Sentence denotes CONCLUSIONS:
T14 1284-1460 Sentence denotes The D614 G mutation in the G clade induced the flexibility of S-protein, resulting in increased furin binding which may enhance S-protein cleave and infiltration of host cells.
T15 1461-1534 Sentence denotes As such, SARS-CoV-2 D614 G mutation may result in a more virulent strain.