LitCovid-sentences  

PubMed:32735992 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-70 Sentence denotes Design of an engineered ACE2 as a novel therapeutics against COVID-19.
T2 71-319 Sentence denotes The interaction between the angiotensin-converting enzyme 2 (ACE2) and the receptor binding domain (RBD) of the spike protein from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a pivotal role in virus entry into the host cells.
T3 320-493 Sentence denotes Since recombinant ACE2 protein has been suggested as an anti-SARS-CoV-2 therapeutic agent, this study was conducted to design an ACE2 protein with more desirable properties.
T4 494-596 Sentence denotes In this regard, the amino acids with central roles in enzymatic activity of the ACE2 were substituted.
T5 597-737 Sentence denotes Moreover, saturation mutagenesis at the interaction interface between the ACE2 and RBD was performed to increase their interaction affinity.
T6 738-877 Sentence denotes The best mutations to increase the structural and thermal stability of the ACE2 were also selected based on B factors and mutation effects.
T7 878-1048 Sentence denotes The obtained resulted revealed that the Arg273Gln and Thr445Gly mutation have drastically reduced the binding affinity of the angiotensin-II into the active site of ACE2.
T8 1049-1150 Sentence denotes The Thr27Arg mutation was determined to be the most potent mutation to increase the binding affinity.
T9 1151-1244 Sentence denotes The Asp427Arg mutation was done to decrease the flexibility of the region with high B factor.
T10 1245-1388 Sentence denotes The Pro451Met mutation along with the Gly448Trp mutation was predicted to increase the thermodynamic stability and thermostability of the ACE2.
T11 1389-1535 Sentence denotes The designed therapeutic ACE2 would have no enzymatic activity while it could bear stronger interaction with Spike glycoprotein of the SARS-CoV-2.
T12 1536-1640 Sentence denotes Moreover, decreased in vivo enzymatic degradation would be anticipated due to increased thermostability.
T13 1641-1755 Sentence denotes This engineered ACE2 could be exploited as a novel therapeutic agent against COVID-19 after necessary evaluations.