LitCovid-sentences  

PubMed:32727845 JSONTXT 41 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-132 Sentence denotes Identification of an anti-SARS-CoV-2 receptor binding domain directed human monoclonal antibody from a naïve semi-synthetic library.
T2 133-357 Sentence denotes There is a desperate need for safe and effective vaccines, therapies and diagnostics for SARS-CoV-2, the development of which will be aided by the discovery of potent and selective antibodies against relevant viral epitopes.
T3 358-517 Sentence denotes Human phage display technology has revolutionized the process of identifying and optimizing antibodies, providing facile entry points for further applications.
T4 518-628 Sentence denotes Here in, we use this technology to search for antibodies targeting the receptor binding domain (RBD) of CoV-2.
T5 629-805 Sentence denotes Specifically, we screened a naïve human semi-synthetic phage library against RBD, leading to the identification of a high-affinity single chain fragment variable region (scFv).
T6 806-889 Sentence denotes The scFv was further engineered into two other antibody formats (scFv-Fc and IgG1).
T7 890-1016 Sentence denotes All the three antibody formats showed high binding specificity to CoV-2 RBD and the spike antigens in different assay systems.
T8 1017-1145 Sentence denotes Flow cytometry analysis demonstrated specific binding of the IgG1 format to cells expressing membrane bound CoV-2 spike protein.
T9 1146-1310 Sentence denotes Docking studies revealed that the scFv recognizes an epitope that partially overlaps with angiotensin converting enzyme 2 (ACE2)-interacting sites on the CoV-2 RBD.
T10 1311-1565 Sentence denotes Given its high specificity and affinity, we anticipate that these anti-CoV-2 antibodies will be useful as valuable reagents for accessing the antigenicity of vaccine candidates, as well as developing antibody-based therapeutics and diagnostics for CoV-2.