| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T176 |
0-4 |
Sentence |
denotes |
4.7. |
| T177 |
5-65 |
Sentence |
denotes |
Reverse-Phase Chromatography (RPC) and ESI Mass Spectrometry |
| T178 |
66-319 |
Sentence |
denotes |
Directly after the CEX chromatography, a 200 µl sample of PAS-Tα1 or PAS-Tα1 was adjusted to 2% v/v acetonitrile, 0.1% v/v formic acid and applied to a Resource RPC 1 mL column (GE Healthcare) equilibrated with 2% v/v acetonitrile, 0.1% v/v formic acid. |
| T179 |
320-542 |
Sentence |
denotes |
The protein was eluted using a concentration gradient of up to 80% v/v acetonitrile, 0.1% v/v formic acid over 20 column volumes at a flow rate of 2 mL/min while spectrophotometrically monitoring protein elution at 225 nm. |
| T180 |
543-721 |
Sentence |
denotes |
The eluted protein fraction was directly injected into a maXis quadrupole time of flight (Q-TOF) instrument (Bruker Daltonics, Bremen, Germany) operated in the positive ion mode. |
| T181 |
722-795 |
Sentence |
denotes |
Raw data was deconvoluted using the MaxEntX algorithm (Bruker Daltonics). |
