| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T141 |
0-4 |
Sentence |
denotes |
4.2. |
| T142 |
5-54 |
Sentence |
denotes |
Bacterial Production of Recombinant PASylated Tα1 |
| T143 |
55-252 |
Sentence |
denotes |
The BL21 derivative E. coli strain NEBexpress® (New England Biolabs, Frankfurt am Main, Germany) was transformed with the plasmids pASK75-Tα1-PAS#1(600)/RimJ or pASK75-MP-PAS#1(600)-Tα1 from above. |
| T144 |
253-408 |
Sentence |
denotes |
Two milliliters of Terrific Broth supplemented with 100 µg/mL ampicillin (TBamp) was inoculated with a single colony and grown overnight at 37 °C, 170 rpm. |
| T145 |
409-546 |
Sentence |
denotes |
This overnight culture was used to inoculate a 50 mL preculture, which was grown under the same conditions until OD550 = 1.1 was reached. |
| T146 |
547-671 |
Sentence |
denotes |
Subsequently, the 50 mL preculture was transferred into 2 L TBamp and incubated in a 5 L baffled flask at 37 °C and 100 rpm. |
| T147 |
672-820 |
Sentence |
denotes |
After reaching OD550 = 0.6, the temperature was decreased to 26 °C and the cells were subsequently induced at OD550 = 1 with 200 µg/mL aTc for 15 h. |
