| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T119 |
0-28 |
Sentence |
denotes |
Specificity of RT-LAMP assay |
| T120 |
29-262 |
Sentence |
denotes |
Following this, to examine the specificity of the RT-LAMP assay, we performed the assay using RNAs extracted from SARS-CoV, which is the most closely related human coronavirus to SARS-CoV-2, and SARS-CoV-2 (n = 2 for each viral RNA). |
| T121 |
263-417 |
Sentence |
denotes |
The RT-LAMP did not detect SARS-CoV RNA regardless of the primer set used, while SARS-CoV-2 RNA was successfully detected with both primer sets (Table 3). |
| T122 |
418-572 |
Sentence |
denotes |
We confirmed the presence of SARS-CoV RNA using RT-qPCR with WHO primer (Table 3, Ct = 27.5 (7,780 copies) and 27.7 (6,890 copies) in duplicated samples). |
| T123 |
573-653 |
Sentence |
denotes |
These results indicate that our RT-LAMP assay is highly specific for SARS-CoV-2. |
| T124 |
654-692 |
Sentence |
denotes |
Table 3 Specificity of RT-LAMP assay. |
| T125 |
693-721 |
Sentence |
denotes |
Primer set SARS SARS-CoV-2 |
| T126 |
722-759 |
Sentence |
denotes |
RT-LAMP(min) ORF1b-1 - - + (11.5) |
| T127 |
760-783 |
Sentence |
denotes |
ORF1b-2 - - + (9.75) |
| T128 |
784-821 |
Sentence |
denotes |
RT-qPCR (Ct) WHO + (27.5) + (27.7) |
