LitCovid-sentences  

PMC:7668588 / 10736-12177 JSONTXT 2 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T89 0-59 Sentence denotes Sensitivity of RT-LAMP assay using in vitro synthesized RNA
T90 60-205 Sentence denotes We designed two SARS-CoV-2 specific LAMP primer sets that targeted conserved sequences in the ORF1b region (name: ORF1b-1 and ORF1b-2) (Table 1).
T91 206-397 Sentence denotes To examine the sensitivity of the RT-LAMP assay for SARS-CoV-2, serial 10-fold dilutions of in vitro synthesized RNA possessing the target sequence of Wuhan-Hu-1 strain were used as template.
T92 398-582 Sentence denotes All (6/6) or 83% (5/6) of the reactions with 50 copies of RNA yielded positive results with ORF1b-1 (RT-LAMP/ORF1b-1) or ORF 1b-2 primer sets (RT-LAMP/ORF1b-2), respectively (Table 2).
T93 583-681 Sentence denotes Additionally, RT-LAMP/ORF1b-1 could detect samples containing 5 RNA copies once in six replicates.
T94 682-818 Sentence denotes Moreover, both RT-LAMP/ORF1b-1 and RT-LAMP/ORF1b-2 could detect 500 RNA copies within 8 min and 50 RNA copies within 11.8 min (Table 2).
T95 819-944 Sentence denotes These results indicate that this RT-LAMP assay can be used as a rapid and sensitive test for the detection of SARS-CoV-2 RNA.
T96 945-1002 Sentence denotes Table 2 Sensitivity and detection time of RT-LAMP assay.
T97 1003-1010 Sentence denotes ORF1b-1
T98 1011-1045 Sentence denotes Copies/reaction 500 50 5 Water
T99 1046-1072 Sentence denotes Time (min) 7.5 9.0 - -
T100 1073-1090 Sentence denotes 7.3 8.8 10.5 -
T101 1091-1106 Sentence denotes 8.0 11.8 - -
T102 1107-1119 Sentence denotes 6.8 9.3 -
T103 1120-1134 Sentence denotes 6.8 9.0 - -
T104 1135-1149 Sentence denotes 7.3 8.5 - -
T105 1150-1173 Sentence denotes Average 7.3 9.4 - -
T106 1174-1192 Sentence denotes SD 0.4 1.1 - -
T107 1193-1221 Sentence denotes Positive 6/6 6/6 1/6 0/6
T108 1222-1229 Sentence denotes ORF1b-2
T109 1230-1265 Sentence denotes Copies/ reaction 500 50 5 Water
T110 1266-1293 Sentence denotes Time (min) 8.0 11.8 - -
T111 1294-1306 Sentence denotes 7.8 - - -
T112 1307-1322 Sentence denotes 7.3 11.5 - -
T113 1323-1337 Sentence denotes 7.5 9.0 - -
T114 1338-1352 Sentence denotes 7.3 9.5 - -
T115 1353-1368 Sentence denotes 6.8 10.3 - -
T116 1369-1393 Sentence denotes Average 7.4 10.4 - -
T117 1394-1412 Sentence denotes SD 0.4 1.1 - -
T118 1413-1441 Sentence denotes Positive 6/6 5/6 0/6 0/6