LitCovid-sentences  

PMC:7650537 / 9623-12674 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T74 0-4 Sentence denotes 2.2.
T75 5-43 Sentence denotes Investigated SARS-CoV-2 Antibody Tests
T76 44-724 Sentence denotes Seven SARS-CoV-2 IgG assays (Abbott SARS-CoV-2 IgG, Wiesbaden, Germany; DiaSorin Liaison® SARS-CoV-2 S1/2 IgG, DiaSorin, Dietzenbach, Germany; Epitope EDI™ Novel Coronavirus COVID-19 IgG ELISA Kit, Epitope Diagnostics, San Diego, CA, USA; Euroimmun Anti-SARS-CoV-2 ELISA (IgG), EUROIMMUN AG, Lübeck, Germany; Mikrogen recomWell SARS-CoV-2 IgG, Mikrogen GmbH, Neuried, Germany; SARS-CoV-2 ViraChip® IgG Test, Viramed Biotech AG, Planegg, Germany; and SERION ELISA agile SARS-COV-2 IgG, Institute Virion-Serion GmbH, Würzburg, Germany), as well as one total SARS-CoV-2 antibody test (Roche Elecsys Anti-SARS-CoV-2, Roche Diagnostics, Mannheim, Germany), were included in this study.
T77 725-947 Sentence denotes The recombinant antigens of these tests cover the N protein (Abbott, Epitope, Mikrogen, Roche) and the entire S protein (Virion-Serion), as well as its S1 domain alone (Euroimmun) or together with the S2 domain (DiaSorin).
T78 948-1149 Sentence denotes The SARS-CoV-2 ViraChip® IgG test kit uses the purified viral surface antigens S1 and S2 and the N antigen, which are all presented separately at a defined position on a nitrocellulose film (Table S1).
T79 1150-1491 Sentence denotes All tests were conducted strictly following the recommendations of the manufacturers on an Architect or Alinity machine (Abbott), a Liaison XL (DiaSorin), or a Cobas e 411 (Roche) or, for the assays of Epitope, Euroimmun, Mikrogen Viramed, and Virion-Serion, on the BEP 2000 system (Siemens Healthcare GmbH, Erlangen, Germany), respectively.
T80 1492-1601 Sentence denotes The ViraChip® test was then evaluated automatically by help of a ViraChip® reader and the ViraChip® Software.
T81 1602-1660 Sentence denotes All borderline/gray zone results were counted as positive.
T82 1661-1795 Sentence denotes Furthermore, raw data from seven assays were converted into relative indices according to the decision limits set by the manufacturer.
T83 1796-1929 Sentence denotes Thereby, a signal (S)/cut-off (CO) value of <1 was valued as negative and ≥1 as positive, which corresponds to a previous study [22].
T84 1930-2073 Sentence denotes Results of the SARS-CoV-2 ViraChip® IgG could not be converted into relative indices and were only given qualitatively as negative or positive.
T85 2074-2213 Sentence denotes The proportion of correctly identified samples per test relative to the chosen reference method was calculated and given as assay accuracy.
T86 2214-2379 Sentence denotes The convalescent sera were also tested with and without avidity reagent (contains urea in denaturating concentrations) in two versions of an IgG line assay (blot v1:
T87 2380-2449 Sentence denotes Mikrogen recomLine Coronavirus IgG (Avidität), prototype and blot v2:
T88 2450-2500 Sentence denotes Mikrogen recomLine SARS-CoV-2 IgG (Avidität)/RUO).
T89 2501-2724 Sentence denotes The first version is based on the N protein of the human coronaviruses (HCoVs) 229E, HKU1, NL63, and OC43, which are used separately as antigens, and on the N protein of the “classic” SARS-CoV from 2002/2003 and SARS-CoV-2.
T90 2725-2885 Sentence denotes The second improved version still uses the N proteins of the aforementioned HCoVs and of SARS-CoV-2 but has been expanded to include the S1 antigen and its RBD.
T91 2886-2941 Sentence denotes A Dynablot Plus system was used to process all samples.
T92 2942-3051 Sentence denotes Blots were then evaluated automatically with a BLOTrix reader and the recomScan software (all from Mikrogen).