LitCovid-sentences  

PMC:7650537 / 7011-9294 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T54 0-130 Sentence denotes A plaque reduction neutralization assay (PRNT) using a SARS-CoV-2 isolate designated as Kiel M16502/2020 and Vero cells (order no.
T55 131-265 Sentence denotes 605372, CLS Cell Lines Service GmbH, Eppelheim, Germany) was included as a further reference for the calculation of the sensitivities.
T56 266-326 Sentence denotes All sera classified as reactive in the PRNT were considered.
T57 327-381 Sentence denotes This also covered follow-up sera (reference method 2).
T58 382-509 Sentence denotes Testing was done under biosafety level 3 conditions and was in accordance to previous reports [20,21] with minor modifications.
T59 510-605 Sentence denotes One to two days before infection, 1.0 × 105 cells were seeded into each well of 48-well plates.
T60 606-691 Sentence denotes These were then incubated under standard conditions until the cells became confluent.
T61 692-1051 Sentence denotes Directly prior to the PRNT, patient sera were heat-inactivated at 56 °C for 30 min and then diluted from 1:10 to 1:1280 in cell culture medium consisting of Dulbecco’s modified Eagle’s medium (DMEM) (Bio&SELL GmbH, Feucht, Germany) supplemented with 3.7 g/L NaHCO3, 4.5 g/L glucose, 2 mM L-glutamine, and 1% (v/v) Penicillin/Streptomycin/Fungi-Mix (Bio&SELL).
T62 1052-1231 Sentence denotes A serum dilution series was made by mixing 50 µL of each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C.
T63 1232-1419 Sentence denotes The cells were washed with phosphate-buffered saline (Bio&SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker.
T64 1420-1578 Sentence denotes Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v).
T65 1579-1770 Sentence denotes After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol.
T66 1771-1812 Sentence denotes The stained plates were photo-documented.
T67 1813-1946 Sentence denotes All dilution steps were tested in quadruplicates, and plaque formation was compared to an untreated cell control and a virus control.
T68 1947-2118 Sentence denotes A serum dilution >1:10, which prevented plaque formation in at least 50% of the wells compared to the virus control, was classified as reactive and probably as protective.
T69 2119-2177 Sentence denotes The reciprocal titers were used to visualize the kinetics.
T70 2178-2283 Sentence denotes If an exact titer could not be given, a geometric mean value was calculated from the two adjacent titers.