LitCovid-sentences  

PMC:7650537 / 20232-24156 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
T163 0-235 Sentence denotes With respect to the previous positive SARS-CoV-2 PCR result considered as the reference of first choice, following a recent suggestion [19], assay sensitivities were found to be relatively high and varied from 80.8% to 96.2% (Table 1).
T164 236-328 Sentence denotes This finding is in accordance to other studies [21,22,24,25,26] and to a meta-analysis [23].
T165 329-593 Sentence denotes Six (23.1%; five, 19.2%, if the positive retesting result of the initial serum from patient 4 is taken into account; for details, see below) of the 26 SARS-CoV-2-infected patients did not develop SARS-CoV-2-neutralizing antibodies, as demonstrated by a PRNT ≤1:10.
T166 594-786 Sentence denotes Among them were two individuals who were only recognized as IgG-positive in the Abbott and Virion-Serion assays and were partially reactive in the ViraChip® and the IgG immunoblots (Figure 1).
T167 787-869 Sentence denotes This confirms a previous report of a high sensitivity of the Abbott IgG test [27].
T168 870-1070 Sentence denotes The absence of neutralizing antibodies in several COVID-19 patients was also reported by others [28] and may be associated with the mild course of the infection mainly observed in outpatient patients.
T169 1071-1264 Sentence denotes However, since no clinical data were available to us, a severe underlying disease or a serious course of the SARS-CoV-2 infection cannot be ruled out in the individuals of our study collective.
T170 1265-1379 Sentence denotes Recently, presence of neutralizing antibodies was found to be associated with protection against reinfection [29].
T171 1380-1563 Sentence denotes If the PRNT was chosen as another reference (Figure S1), sensitivities varied from 88.9% to 96.3% (Table 1), which is largely comparable to the results obtained with the first method.
T172 1564-1797 Sentence denotes These sensitivities can be even higher, as retesting of the initial serum from patient 4 in the second part of our study revealed the presence of low-level neutralizing antibodies (1:10 vs. 1:20–1:40; Figure 5 and Figures S1 and S2).
T173 1798-1992 Sentence denotes With respect to a sample from patient 1 taken six days after the PCR, two S-based assays failed to detect SARS-CoV-2 IgG, while all assays that used the N as an epitope were reactive (Figure 1).
T174 1993-2104 Sentence denotes Thus, usage of the latter may lead to the detection of IgG/total antibodies at an earlier stage post-infection.
T175 2105-2263 Sentence denotes Furthermore, a lack of reactivity in some assays using the S antigen does not necessarily mean that SARS-CoV-2-neutralizing antibodies are missing (Figure 1).
T176 2264-2333 Sentence denotes Seroconversion was clearly demonstrated in three patients (Figure 1).
T177 2334-2466 Sentence denotes The majority of SARS-CoV-2 IgG in sera from SARS-CoV-2-infected patients were verified by both versions of an immunoblot (Figure 1).
T178 2467-2572 Sentence denotes The latter can be used to differentiate the antibody reactivities against individual SARS-CoV-2 antigens.
T179 2573-2743 Sentence denotes The specificities of the SARS-CoV-2 IgG/total antibody tests are at comparatively high levels between 96.0% and 100.0%, as has also been reported by others [21,22,24,26].
T180 2744-2952 Sentence denotes Interestingly, one sample collected in the winter 2018/2019 was found to be reactive in the Mikrogen, Roche, and Viramed assays, as well as in the prototype immunoblot, but could not be confirmed by the PRNT.
T181 2953-3157 Sentence denotes For all other 99 archived samples, a random pattern of rare, isolated reactivity was demonstrated, and none was confirmed as possessing SARS-CoV-2-neutralizing antibodies by PRNT (Figure 2 and Figure S1).
T182 3158-3266 Sentence denotes Potential cross-reactivity with the HCoVs responsible for common colds may therefore not be a major problem.
T183 3267-3472 Sentence denotes The analysis of the immunoblot results shows, however, that sera from SARS-CoV-2-infected patients may simultaneously react with the antigens from HCoVs 229E, HKU-1, NL63, OC43, and SARS-CoV-2 (Figure S3).
T184 3473-3663 Sentence denotes Due to the currently estimated low prevalence of SARS-CoV-2 IgG in the overall German population, even high specificities of 96–98% would produce a relevant number of false positive results.
T185 3664-3817 Sentence denotes Thus, such assays, which all show an accuracy over 93%, should be preferentially used for the testing of patients with a history of a probable infection.
T186 3818-3924 Sentence denotes In the case of doubt, the implementation of the labor-intensive and time-consuming PRNT may be considered.