| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T51 |
0-204 |
Sentence |
denotes |
At 24 h postinfection (hpi), both PDCoV- and mock-infected IPEC-J2 cells grown in T25 flasks (∼5 × 106 cells/flask) were rinsed twice with prechilled PBS, and then harvested with disposable cell scrapers. |
| T52 |
205-315 |
Sentence |
denotes |
Three flasks of each group (PDCoV or mock) were harvested and used as three independent biological replicates. |
| T53 |
316-525 |
Sentence |
denotes |
After centrifugation at 300g for 10 min, the cell pellets from each flask were lysed with 800 μL of RIPA lysis buffer (Beyotime, Shanghai, China) containing 1% SDS, 8 M urea and protease inhibitors (Beyotime). |
| T54 |
526-631 |
Sentence |
denotes |
The cell lysates were further sonicated on ice for 5 min with 10 s bursts and 10 s pauses between cycles. |
| T55 |
632-755 |
Sentence |
denotes |
After centrifugation for 20 min at 12 000g and 4 °C, the supernatant was collected and used as the total cellular proteins. |
| T56 |
756-884 |
Sentence |
denotes |
The concentration of protein samples was determined using a Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL, USA). |
