LitCovid-sentences  

PMC:7640975 / 9280-10725 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T46 82-210 Sentence denotes After a 48-h cultivation at 37 °C with 5% CO2, the cells were fixed with prechilled 100% ethanol for 15 min at room temperature.
T47 211-309 Sentence denotes After washing with PBS, the cells were probed with the mAb 1A3 (1:5000 dilution) at 37 °C for 1 h.
T48 310-487 Sentence denotes Following another washing step, 1000-fold-diluted Alexa Fluor 488-conjugated goat antimouse IgG (Thermo Fisher Scientific) was added to the cells and incubated at 37 °C for 1 h.
T49 488-599 Sentence denotes After washing thrice with PBS, cells were observed with an Eclipse Ci-S microscope (Nikon Corp., Tokyo, Japan).
T50 601-655 Sentence denotes 2.5 Protein Extraction, Digestion, and iTRAQ Labeling
T51 656-860 Sentence denotes At 24 h postinfection (hpi), both PDCoV- and mock-infected IPEC-J2 cells grown in T25 flasks (∼5 × 106 cells/flask) were rinsed twice with prechilled PBS, and then harvested with disposable cell scrapers.
T52 861-971 Sentence denotes Three flasks of each group (PDCoV or mock) were harvested and used as three independent biological replicates.
T53 972-1181 Sentence denotes After centrifugation at 300g for 10 min, the cell pellets from each flask were lysed with 800 μL of RIPA lysis buffer (Beyotime, Shanghai, China) containing 1% SDS, 8 M urea and protease inhibitors (Beyotime).
T54 1182-1287 Sentence denotes The cell lysates were further sonicated on ice for 5 min with 10 s bursts and 10 s pauses between cycles.
T55 1288-1411 Sentence denotes After centrifugation for 20 min at 12 000g and 4 °C, the supernatant was collected and used as the total cellular proteins.