| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T44 |
0-8 |
Sentence |
denotes |
2.4 IFA |
| T45 |
9-162 |
Sentence |
denotes |
When IPEC-J2 cells grown in 96-well microplates reached ∼80% confluence, they were mock infected or infected with PDCoV at an MOI of 0.1 TCID50 per cell. |
| T46 |
163-291 |
Sentence |
denotes |
After a 48-h cultivation at 37 °C with 5% CO2, the cells were fixed with prechilled 100% ethanol for 15 min at room temperature. |
| T47 |
292-390 |
Sentence |
denotes |
After washing with PBS, the cells were probed with the mAb 1A3 (1:5000 dilution) at 37 °C for 1 h. |
| T48 |
391-568 |
Sentence |
denotes |
Following another washing step, 1000-fold-diluted Alexa Fluor 488-conjugated goat antimouse IgG (Thermo Fisher Scientific) was added to the cells and incubated at 37 °C for 1 h. |
| T49 |
569-680 |
Sentence |
denotes |
After washing thrice with PBS, cells were observed with an Eclipse Ci-S microscope (Nikon Corp., Tokyo, Japan). |
