LitCovid-sentences  

PMC:7640975 / 6453-7822 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T24 0-48 Sentence denotes The PDCoV CHN-HN-1601 strain (GenBank access no.
T25 49-189 Sentence denotes MG832584) used in this study was isolated from the intestinal contents of a piglet with diarrhea using porcine kidney LLC-PK1 cells in 2017.
T26 190-319 Sentence denotes The eighth passage of PDCoV with a titer of 108.45 50% tissue culture infectious dose (TCID50)/mL was used for virus inoculation.
T27 320-450 Sentence denotes IPEC-J2 cells were generously provided by Prof. Yongchang Cao (School of Life Sciences, Sun Yat-Sen University, Guangzhou, China).
T28 451-809 Sentence denotes The cells were cultivated in Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12) (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), 5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium (Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Solarbio Life Sciences, Beijing, China).
T29 810-914 Sentence denotes Cells were cultured at 37 °C in a humidified CO2 incubator (Thermo Fisher Scientific, Waltham, MA, USA).
T30 915-1028 Sentence denotes The monoclonal antibody (mAb), 1A3, raised against the PDCoV nucleocapsid protein was prepared in our laboratory.
T31 1029-1159 Sentence denotes Mouse anti-β-actin mAb, mouse anti-IFIT1 and rabbit anti-ANAPC7 polyclonal antibodies were bought from Abcam (Cambridge, MA, USA).
T32 1160-1278 Sentence denotes Horseradish peroxidase (HRP)-conjugated goat antimouse/rabbit immunoglobulin G (IgG) were obtained from Sigma-Aldrich.
T33 1279-1369 Sentence denotes Alexa Fluor 488-conjugated goat antimouse IgG was the product of Thermo Fisher Scientific.